It only makes sense that a person’s nutritional needs change as they go through life from childhood through adulthood. Older people need more protein due to the body no longer being able to process the macronutrient efficiently.In the study, the sweet spot for moderate protein consumption was between 25% and 35% of a mouse’s daily diet.A new study in mice suggests that consuming a moderate amount of protein may be most conducive to improved metabolic health.Interestingly, more robust upregulation of Redd1 than atrogenes was observed in different muscle types in both sexes, suggesting it may serve as a reliable mRNA marker for C26-induced CC.ĪCKNOWLEDGEMENTS: This study was funded by NIH Grant R01 AR075794-02.Share on Pinterest Moderate protein intake from a range of plant- or animal-based sources could help you live longer, a new study suggests. CONCLUSION: mRNA targets for atrogenes and Redd1 were upregulated as tumors develop. Deptor was 1.5 fold lower in 15d than 25d (p<0.05). In female soleus, Redd1, Atrogin, and MuRF were greater in 25d than other groups (2-5.7 fold p<0.01). Atrogin and MuRF were greater in 25d than other groups (1.3-2 fold p<0.01). Deptor was ~1.3 fold lower in 15d than PBS and 25d (~1.3 fold p<0.05). In male soleus, Redd1 was greater in 25d than PBS, 10d and 15d (1.7-3.4 fold p<0.01). Atrogin was 3.6 fold greater in 20d than 15d (p<0.05). In female Gast, Redd1 was greater in 20d than PBS and 10d (2.7-6 fold p<0.05). In male Gast, Redd1, Atrogin, and MuRF were greater in 25d than other groups. Atrogin was greater in 25d than other groups (1.6-2.4 fold p<0.05). Deptor was 2.7 fold greater in 25d than PBS (P<0.05). In female TA, Redd1 was 2.2-3.5 fold greater in 25d than PBS and 15d (p<0.05). RESULTS: In male TA, Redd1, Atrogin, and MuRF were greater in 25d than other groups (2.2-8.4 fold p<0.01). A one-way ANOVA was utilized within each sex as the global analysis with α=0.05. All tissues were prepared for qRT-PCR to determine mRNA contents for protein turnover. Muscle tissues (Tibialis anterior TA, Gastrocnemius Gast, and Soleus) were collected at designated time points. METHODS: 64 male and 59 female BALB/c mice were injected subcutaneously with C26 colon carcinoma cells or PBS at 8-wk old and tumors were allowed to develop for 10, 15, 20, and 25 days. PURPOSE: To assess the role of anabolic repressors and atrogenes during the development of CC between sexes. However, it is unknown if CC affects these regulators differently during the development of CC between biological sexes in different muscle types. Prior studies revealed anabolic repressors ( Redd1 & Deptor) and atrogenes ( Atrogin & MuRF), as key regulators of CC in rodents. Preclinical CC studies have focused on potential therapeutic targets to ameliorate cachectic phenotypes. Greene, FACSM 1ġUniversity of Arkansas, Fayetteville, ArkansasĬancer cachexia (CC) is a wasting syndrome characterized by an ongoing loss of muscle mass that negatively affects quality of life for cancer patients. Saling 1, Anthony Campitelli 1, Tyrone A. William Deaver 1, Francielly Morena da Silva 1, Ana Regina Cabrera 1, Eleanor R.
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